TABLE 1.
Primers used for AAV2 HI loop mutant capsid generation
| Primer | Forward | Reverse |
|---|---|---|
| AAV2 HI−/− | 5′ TCC TTC ATC ACA CAG TAC TCC ACG GGA CAG G 3′ | 5′ AGG ATT CGC AGG TAC CGG GGT GTT CTT GAT GAG 3′ |
| AAV2 poly-glycine | 5′ GGA GGA GGA GGA GGA GGA GGA GGA GGA GGA TCC TTC ATC ACA CAG TAC TCC ACG GGA CAG G 3′ | TCC TCC TCC TCC TCC TCC TCC TCC TCC TCC AGG ATT CGC AGG TAC CGG GGT GTT CTT GAT GAG 3′ |
| AAV2 poly-glycine F661 | 5′ GCG AAT CCT GGA GGA GGA TTC GGA GGA GGA GGA GGA 3′ | NA |
| AAV2 poly-glycine K665 | 5′ GGA GGA GGA GGA GGA AAG GGA GGA TCC TTC ATC 3′ | NA |
| AAV2 poly-glycine F666 | 5′ GGA GGA GGA GGA GGA TTT GGA TCC TTC ATC ACA CAG 3′ | NA |
| pXSA for HI loop serotype swaps | (Sbf1) 5′ AGA GAT GTG TAC CTG CAG GGG CCC ATC TGG 3′ | NA |
| (Afe1) 5′ AAG GAA AAC AGC AAG CGC TGG AAT CCC GAA 3′ | ||
| AAV5 HI loop | 5′ GAT CCT GCA GGG ACC CAT CTG GGC CAA GAT C 3′ | 5′ GCT TGG AGT TTT CCT TCT TGA GCT CCC AC 3′ |
| AAV4 HI loop | 5′ GAT CCT GCA GGG TCC CAT TTG GGC CAA GAT T 3′ | 5′ GTT TGG ACC GCT CCT TCT GGA TCT CCC 3′ |
| AAV2 HI5 TTSF | 5′ CCC GGA AAT ATC ACC ACC AGC TTC TCG GAC GTG 3′ | NA |
| pAge1 Nhel | (Age1) 5′ CTC ATC AAG AAC ACA CCG GTA CCT GCG 3′ | NA |
| (Nhe1) 5′ GCG GCA AAG TTT GCT AGC TTC ATC ACA CAG TAC TCC 3′ | ||
| AAV2 SIGYPLP | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT AGC ATT GGT TAT CCT CTT CCT AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT AGG AAG AGG ATA ACC AAT GCT AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 VNTANST | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT GTT AAT ACT GCT AAT AGC ACT AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT AGT GCT ATT AGC AGT ATT AAC AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 QPEHSST | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT CAA CCT GAA CAT AGC AGC ACT AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT AGT GCT GCT ATG TTC AGG TTG AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 RGD 658 | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT CGA GGA GAC TTC AGT GCG GCA AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT TGC CGC ACT GAA GTC TCC TCG AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 RGD 660 | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT TCG ACC CGA GGA GAC GCG GCA AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT TGC CGC GTC TCC TCG GGT CGA AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 RGD 662 | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT TCG ACC ACC TTC CGA GGA GAC AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT GTC TCC TCG GAA GGT GGT CGA AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 RGD 663 | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT TCG ACC ACC TTC AGT CGA GGA GAC TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA GTC TCC TCG ACT GAA GGT GGT CGA AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 SGRGDS | 5′ AAG AAC ACA CCG GTA CCT GCG AAT CCT TCG GGA CGA GGA GAC TCG GCG AAG TTT GCT AGC TTC ATC 3′ | 5′ GAT GAA GCT AGC AAA CTT CGC CGA GTC TCC TCG TCC CGA AGG ATT CGC AGG TAC CGG TGT GTT CTT 3′ |
| AAV2 F661G | 5′ GCG AAT CCT TCG ACC ACC GGC AGT GCG GCA AAG TTT GCT TCC 3′ | NA |