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. 2008 Mar 19;82(11):5220–5233. doi: 10.1128/JVI.02515-07

FIG. 2.

FIG. 2.

HSV-2 UL56 interacts with Nedd4 via PY motifs in transfected cells. (A) UL56WT interacts with Nedd4. Endogenous Nedd4 was immunoprecipitated (IP) from Vero cells transiently expressing UL56WT. Western blotting (WB) with an anti-UL56 serum detected UL56 in the precipitants (second panel from the top, lane 4). Controls to detect the levels of immunoprecipitation (top, lanes 3 to 5) and expression (Nedd4, top, lanes 1 and 2; UL56, second panel from the top, lanes 1and 2) were blotted with the corresponding antibodies. (B) The UL56AY mutant does not interact with Nedd4. Immunoprecipitation and Western blot analyses were performed as described for panel A. UL56WT but not UL56AY was detected in the immunoprecipitants. β-Actin (in whole-cell lysates [WCL]) and IgG heavy chain (H) (in IP) were used as loading controls.