FIG. 7.

Seed sequence specificity of miR-155 and Western blot analysis of BACH1 expression in miR-155 expressing cells. (A) 3′ UTR experiments were carried out as for Fig. 6. “Wt” represents a 3′ UTR reporter containing 40 bp of wild-type sequences including the BACH1 10-mer miR-155 site, and “Mut” represents the 3′ UTR reporter containing the same sequences with a 6-bp mutation in the miR-155 seed sequence. (B) Nuclear extracts were generated from the indicated cells, and equal amounts of total protein were loaded in all lanes. Western blots were probed with the BACH1 and actin antibodies.