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Immunogenicity of rAd5 vaccination with in vivo EP. Groups of BALB/c mice (four animals/group) were immunized with 109, 107, or 105 vp gp120 rAd5 expressing Env IIIB gp120 alone or with needle EP. Cellular immune responses were assessed by Dd/P18 tetramer binding assays (A) and Env pooled peptide and P18 epitope peptide IFN-γ ELISPOT assays (B) at week 4 after immunization.
