FIG. 2.

Analysis of viral replication in Sf-9 cells cotransfected with vAcΔΔRTPase-PG and lef-4 or ptp/bvp constructs. Cells were transfected with control bacmid DNA (panels A and I) or the double-knockout RTPase bacmid (ΔΔRTPase-PG) alone (panels B and J) or cotransfected with plasmids encoding wt lef-4 (panels C and K), ptp/bvp (panels D and L), lef-4 mutant E9/11A (panels E and M), lef-4 mutant R51A (panels F and N), lef-4 mutant E181/183 (panels G and O), or lef-4 mutant K255A (panels H and P). Cells were analyzed by fluorescence microscopy at 24 h (panels A to H) and 48 h (panels I to P) posttransfection.