FIG. 5.
Pulse-labeling time course of viral protein synthesis. Sf-9 cells were infected with the double-mutant virus vAcΔΔRTPase-PGLEF-4m or the control vAc-PG at an MOI of 5. At different times postinfection, cells were pulse labeled with [35S]methionine-cysteine for 4 h. At the end of the labeling period, detergent-soluble protein extracts were prepared and analyzed on an SDS-10% polyacrylamide gel. The times above each lane represent the harvest time after a 4-h labeling period. Each lane represents 1 × 104 cells. The positions of molecular markers are shown on the left. UI, uninfected cells.