TABLE 1.
MA sequences important for Mo-MLV replication are important for transformation of NIH 3T3 cells by Ab-MLVa
| Virus | Transforming titer (FFU/ml)b
|
|
|---|---|---|
| Expt 1 | Expt 2 | |
| P120 | 3.0 × 103 | 4.6 × 103 |
| G2A | <1 | <1 |
| 54-56A | 6.5 × 101 | 6.5 × 101 |
| 60-62A | 8.0 × 101 | 5.0 × 101 |
| 69-71A | 2.5 × 103 | 1.5 × 103 |
| 85-87A | 1.1 × 102 | 5.3 × 101 |
a
Transformation was assayed by using NIH 3T3 cells (26). Cells were infected with serial dilutions of virus stocks of similar titers. All mutants were tested for transformation in at least two independent experiments.
b
The numbers of transformed foci formed per milliliter of virus added were determined. Values with a less-than symbol (<) represent the limit of detection. FFU, focus-forming units.