Table 10.
Comparison of the transcriptome of human GM-CSF monocyte-derived DCs to that of blood DCs
| Ratio to monocytes | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Probe set ID | Name | Mono | PBMC-MΦ | mo-MΦ | mo-DC | CD16 | BDCA3 | BDCA1 | pDCs |
| Myeloid signature genes | |||||||||
| 222934_s_at | CLEC4E | 2,358 | 0.20 | 0.19 | 0.04 | - | - | 0.05 | - |
| 209930_s_at | NFE2 | 823 | 0.06 | 0.06 | 0.89 | 0.10 | - | 0.06 | - |
| 202974_at | MPP1 | 3,622 | 0.40 | 1.25 | 0.68 | 0.33 | 0.08 | 0.15 | 0.11 |
| 205098_at | CCR1 | 3,528 | 0.76 | 1.63 | 1.83 | 0.03 | - | 0.03 | 0.03 |
| 203535_at | S100A9 | 11,192 | 0.05 | 0.37 | 0.01 | 0.12 | 0.02 | 0.17 | 0.01 |
| 201743_at | CD14 | 8,096 | 0.44 | 1.13 | 0.34 | 0.01 | - | 0.02 | 0.01 |
| 224341_x_at | TLR4 | 1,417 | 0.13 | 1.10 | 0.35 | 0.34 | - | 0.06 | - |
| 203561_at | FCGR2A | 2,946 | 0.18 | 0.80 | 1.36 | 0.85 | - | 0.33 | 0.02 |
| 204924_at | TLR2 | 3,220 | 0.14 | 0.80 | 0.32 | 0.54 | 0.08 | 0.31 | 0.02 |
| 218739_at | ABHD5 | 285 | 0.35 | 0.99 | 0.67 | 0.33 | - | - | - |
| 201089_at | ATP6V1B2 | 3,178 | 2.05 | 2.46 | 1.70 | 0.66 | 0.12 | 0.34 | 0.21 |
| 201631_s_at | IER3 | 2,042 | 0.42 | 1.74 | 0.82 | 0.10 | 0.06 | 0.14 | 0.12 |
| 222218_s_at | PILRA | 2,709 | 0.73 | 1.24 | 1.23 | 1.25 | 0.05 | 0.39 | 0.05 |
| 210423_s_at | SLC11A1 | 1,713 | 0.47 | 0.82 | 0.25 | 1.75 | 0.04 | 0.05 | - |
| 203045_at | NINJ1 | 1,190 | 1.69 | 3.59 | 3.41 | 1.59 | 0.27 | 0.44 | 0.26 |
| 200958_s_at | SDCBP | 11,323 | 0.87 | 1.16 | 0.90 | 0.61 | 0.33 | 0.40 | 0.26 |
| 202917_s_at | S100A8 | 15,661 | 0.02 | 0.41 | 0.01 | 0.11 | 0.01 | 0.27 | 0.03 |
| 217748_at | ADIPOR1 | 2,229 | 0.57 | 0.48 | 1.16 | 0.30 | 0.30 | 0.36 | 0.28 |
| 201669_s_at | MARCKS | 2,340 | 0.84 | 2.57 | 1.57 | 0.65 | 0.16 | 0.20 | - |
| 207697_x_at | LILRB2 | 3,260 | 0.29 | 0.64 | 0.76 | 1.36 | 0.02 | 0.39 | 0.24 |
| 228220_at | FCHO2 | 619 | 4.50 | 4.04 | 3.62 | 0.76 | 0.35 | 0.26 | 0.23 |
| 1553297_a_at | CSF3R | 3,121 | 0.42 | 0.69 | 0.37 | 0.08 | 0.11 | 0.52 | 0.04 |
| 220088_at | C5AR1 | 2,059 | 2.56 | 3.63 | 1.30 | 1.60 | - | 0.03 | 0.04 |
| 212501_at | CEBPB | 3,490 | 3.26 | 3.23 | 3.30 | 1.26 | 0.06 | 0.49 | 0.06 |
| 221698_s_at | CLEC7A | 6,596 | 0.24 | 0.55 | 0.63 | 0.74 | 0.62 | 0.46 | 0.01 |
| 209551_at | YIPF4 | 526 | 0.85 | 1.65 | 1.91 | 0.41 | 0.37 | 0.44 | 0.37 |
| 204204_at | SLC31A2 | 1,933 | 0.94 | 1.14 | 0.69 | 0.76 | 0.10 | 0.22 | 0.03 |
| Pan-DC signature genes | |||||||||
| 206674_at | FLT3 | 221 | - | - | - | - | 24.01 | 12.76 | 9.26 |
| 219256_s_at | SH3TC1 | 395 | 1.02 | 2.73 | 1.12 | 2.01 | 2.22 | 3.01 | 2.86 |
| 218617_at | TRIT1 | 498 | 0.49 | 0.58 | 0.86 | 0.71 | 2.46 | 2.15 | 3.61 |
| 231810_at | BRI3BP | 301 | 0.98 | 1.42 | 1.99 | 0.98 | 2.35 | 2.10 | 2.70 |
| 209139_s_at | PRKRA | 325 | 1.12 | 1.77 | 1.47 | 1.57 | 3.17 | 2.42 | 4.37 |
| 225764_at | ETV6 | 1,097 | 0.43 | 1.13 | 2.00 | 0.75 | 2.04 | 1.78 | 1.48 |
| 208837_at | TMED3 | 595 | 1.50 | 2.81 | 1.64 | 1.46 | 2.91 | 1.98 | 2.94 |
| 219218_at | BAHCC1 | - | - | - | - | - | >1.7 | >1.5 | >4.7 |
| 1552256_a_at | SCARB1 | 151 | 8.98 | 6.58 | 7.21 | - | 2.33 | 1.70 | 5.30 |
| cDC signature genes | |||||||||
| 206298_at | ARHGAP22 | - | >5.8 | >6.5 | >3.1 | - | >6.2 | >4.6 | - |
| 227329_at | BTBD4 | - | >1.6 | >2.8 | >5.8 | - | >9.3 | >8.7 | - |
| 219386_s_at | SLAMF8 | 98 | 24.75 | 38.66 | 23.99 | 0.51 | 15.48 | 5.30 | 0.51 |
| 220358_at | SNFT | 148 | 0.62 | 0.34 | 8.62 | 5.66 | 16.01 | 4.82 | 0.34 |
| 224772_at | NAV1 | 64 | 2.01 | 3.25 | 1.40 | 2.00 | 23.87 | 10.50 | 1.62 |
| 205101_at | CIITA | 481 | 0.29 | 0.12 | 1.09 | 0.48 | 4.51 | 5.28 | 1.43 |
| 218631_at | AVPI1 | - | >18.7 | >31.3 | >64.8 | >1.6 | >3.2 | >7.0 | - |
| 202826_at | SPINT1 | 84 | 4.65 | 7.15 | 8.79 | 0.90 | 2.59 | 2.92 | 0.68 |
| 208660_at | CS | 1,848 | 1.24 | 0.99 | 1.04 | 0.84 | 1.70 | 1.63 | 0.89 |
| APC signature genes | |||||||||
| 203932_at | HLA-DMB | 5,137 | 1.28 | 0.64 | 1.37 | 0.44 | 1.45 | 1.62 | 1.14 |
| 219574_at | MARCH1 | 1,133 | 0.42 | 0.89 | 0.73 | 0.62 | 0.64 | 0.44 | 0.46 |
| 201425_at | ALDH2 | 8,782 | 0.51 | 0.54 | 0.34 | 0.18 | 0.84 | 1.01 | 0.69 |
| 222891_s_at | BCL11A | 310 | 0.98 | 0.34 | 0.50 | 0.74 | 2.40 | 1.73 | 14.23 |
| 205504_at | BTK | 1,372 | 0.29 | 0.47 | 0.64 | 1.13 | 0.58 | 0.75 | 0.81 |
| 202295_s_at | CTSH | 3,755 | 1.76 | 2.37 | 2.09 | 0.56 | 0.63 | 1.57 | 0.31 |
| 209312_x_at | HLA-DRB1 | 12,737 | 1.02 | 0.57 | 1.34 | 1.11 | 1.12 | 1.11 | 1.00 |
| 209619_at | CD74 | 8,540 | 1.49 | 0.86 | 2.12 | 0.73 | 1.33 | 1.34 | 1.11 |
| 210042_s_at | CTSZ | 369 | 0.76 | 1.13 | 17.00 | 1.66 | 2.13 | 2.17 | 1.83 |
| 201560_at | CLIC4 | 2,828 | 0.87 | 0.88 | 1.00 | 0.12 | 0.10 | 0.28 | 0.22 |
| 217388_s_at | KYNU | 3,429 | 1.50 | 1.95 | 0.90 | 0.94 | 0.30 | 0.65 | 0.63 |
| 217118_s_at | C22orf9 | 1,617 | 3.33 | 3.46 | 2.77 | 1.43 | 1.85 | 1.79 | 1.04 |
| 203927_at | NFKBIE | 173 | 3.30 | 9.96 | 3.13 | 1.45 | 1.39 | 2.60 | 1.25 |
| 220998_s_at | UNC93B1 | 847 | 0.60 | 1.31 | 0.97 | 1.31 | 0.99 | 1.06 | 2.27 |
| Non-DC signature genes | |||||||||
| 219243_at | GIMAP4 | 4,384 | 0.15 | 0.11 | 0.19 | 0.27 | - | - | - |
| 221704_s_at | VPS37B | 559 | 0.26 | 0.90 | 0.47 | 0.80 | - | - | - |
| 204891_s_at | LCK | 96 | 1.48 | 0.52 | 0.52 | 0.59 | - | - | - |
| 214582_at | PDE3B | 144 | 2.82 | 2.99 | 2.43 | 0.76 | - | 0.51 | - |
*Average expression across replicates. Genes for which expression between monocyte-derived DCs and blood DCs or blood cDCs varies more than two-fold are shown in bold. mo-DC, monocyte-derived GM-CSF DC; mo-MΦ, monocyte-derived M-CSF macrophages; mono, monocyte; PBMC-MΦ, human peripheral blood mononuclear cell-derived M-CSF macrophages.