Table 1. Comparison of clonogenic cell survival of four colon cancer cell lines with initial DNA damage and initial DNA repair rate assessed using the comet assay.
Clonogenic assay | Comet assay (initial damage)a | Comet assay (damage rejoining)b | |||
---|---|---|---|---|---|
Cell Line | SF2±1 s.e.m.c | Tail moment U Gy−1±1 s.e.m. | d[r2] | Initial (0–15 min) repair half time after 5 Gy (min) | [r2]d |
HT29 | 0.72±0.29 | 2.02±0.13 | 0.992 | 13.7 | 0.83 |
SW480 | 0.69±0.32 | 2.20±0.08 | 0.997 | 10.7 | 0.79 |
SW707 | 0.50±0.19 | 2.64±0.13 | 0.993 | 12.2 | 0.90 |
SW48 | 0.14±0.18 | 3.99±0.10 | 0.999 | 13.7 | 0.87 |
Initial damage per unit dose expressed as the linearly fitted slope of the four observations (0–5 Gy) for each dose–response curve from Figure 3.
Rate of damage rejoining expressed as the slope of the exponential fit to the first three observations (0–15 min) for each curve from Figure 4.
Fraction of cells surviving exposure to 2 Gy.
Correlation coefficient of fit.