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. 2004 Jan 6;90(1):230–235. doi: 10.1038/sj.bjc.6601506

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Copyright © 2004 Cancer Research UK

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Schematic cloning of PS1/2-TAT proteins and transduction efficiency. (A) The PLC-γ1-(SH2)₁ and the PLC-γ1-(SH2)₂ inserts were amplified by RT–PCR and cloned in-frame into the pTAT expression vector. The yielded expression vectors coding for the particular protein were entitled PS1-TAT and PS2-TAT. (B) The uptake of the recombinant PS1/2-TAT fusion proteins into MDA-HER2 cells was analysed by flow cytometry using FITC-labelled PS1/2-TAT fusion proteins. Both PS1-TAT as well as PS2-TAT rapidly transduced into 100% of MDA-HER2 cells, achieving maximum intracellular concentration within 5 min. This figure shows a representative histogram plot of MDA-HER2 cells positively transduced with 2 μM PS2-TAT. Untreated MDA-HER2 cells served as a control.