Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Jan 6;90(1):230–235. doi: 10.1038/sj.bjc.6601506

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The EGF-induced PLC-γ1 activation is blocked by PS2-TAT. (A) The EGF-induced calcium influx was used as a read-out to study the inhibitory capacities of the PS1/2-TAT proteins. Treatment of MDA-HER2 cells with PS2-TAT (2 μM) but not PS1-TAT (2 mM) resulted in a complete blockade of the EGF (100 ng ml⁻¹)-induced calcium influx. U73122 (2 μM) was used as a control. (B) Western Blot analysis was performed to study the mode of action as to how PS2-TAT blocks PLC-γ1 activation. Owing to the higher number of cells that were used for these experiments, the amount of PS1/2-TAT was increased accordingly to 133 μM. PS2-TAT treatment resulted in a markedly reduced PLC-γ1 tyrosine phosphorylation in the MDA-HER2 cell line after 2 min of EGF (100 ng ml⁻¹) stimulation. PS1-TAT, did not interfere with PLC-γ1 tyrosine phosphorylation in MDA-HER2 cells. (C) Confocal laser scanning microscope analysis revealed a translocation of PS2-TAT (2 μM) proteins towards the plasma membrane of MDA-HER2 cells after 2 min of EGF (100 ng ml⁻¹) stimulation, where it is found to be colocalised with the constitutively formed EGFR/c-erbB-2 heterodimer (indicated by c-erbB-2 receptor staining). Bar=10 μm.