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. 2004 Jan 6;90(1):194–199. doi: 10.1038/sj.bjc.6601465

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Copyright © 2004 Cancer Research UK

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Construction of the anti-hScrib antibody using its C-terminus. (A) Structure of hScrib consists of 16 canonical LRRs, followed by LAPSD (LAP-specific domain) and four PDZ domains. We generated an anti-hScrib antibody (anti-hScrib C-terminus) using C-terminus (AA 1208–1630) as antigens. (B) Anti-hScrib C-terminus antibody detected the endogenous hScrib protein with 220 kDa in molecular weight in 293 T cells (lane 1) and in vitro-translated hScrib (lane 2), but not in vitro-translated hDlg by Western blotting (lane 3).