Figure 3.

Recovery of S. enteritidis lpf phase variants from infected mice. Mice were either naïve (A and D) or had been immunized before infection either with a S. typhimurium lpf deletion mutant (TN4) (B and E) or a lpf phase-on culture of S. typhimurium strain TN3 (C and F). Total numbers of bacteria recovered from feces (A–C) are given for individual animals (○). The limit of detection was approximately 0.02 cfu/mg feces. The S. enteritidis lpf phase on/phase off ratios present in the challenge inoculum (solid bar) or recovered from fecal pellets or the spleens (open bars represent mean ± SE) are shown (D–F). In D, bacteria were recovered from the cecum at day 5. The spleen was collected from moribund animals between 5 and 7 days postinfection.