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Indirect immunofluorescence assay showing APY-LUC43 female mosquito salivary glands (A) 10 days after intrathoracic inoculation with saline, (B) 48 hr after inoculation with Anti-luc virus, and (C) 10 days after inoculation with Anti-luc virus. SIN virus infection is confirmed in B and C by use of a primary antibody that recognizes SIN E1 protein (41). DL, distal-lateral lobe; M, medial lobe; PL, proximal-lateral lobe. Magnifications: A, ×200; B and C, ×100.
