Table 1.

K-Rev binds to CRM1 and to itself in vivo

Transfected effector plasmids	Relative activation
GAL4 + K-Rev/VP16	1.0
GAL4/Crm1 only	0.7  ±  0.4
GAL4/Crm1 + K-Rev/VP16	8.2  ±  2.0
GAL4/K-Rev only	0.7  ±  0.1
GAL4/K-Rev + K-Rev/VP16	27.4  ±  2.4

Mammalian two-hybrid assays were performed in 293T cells as described (6). These data (±SEM) represent the average of four experiments and are expressed relative to the culture transfected with plasmids expressing GAL4 and K-Rev/VP16, which was set arbitrarily at 1.0.