Abstract
Detached warty lenticellate bark of a mangrove tree species, Bruguiera gymnorrhiza (L.) Lamk. from Iriomote Island, Okinawa, a subtropical region of Japan, showed development of acetylene reduction activity when incubated in a mineral nutrient solution lacking nitrogen under an atmosphere consisting of 5% O2, 90% N2, and 5% C2H2. The bacteria responsible for nitrogen fixation were isolated from the bark, and their capacity for acetylene reduction and the incorporation of 15N2 into the bacterial cells was confirmed. Four representative strains of the isolates were subjected to taxonomic classification. Two strains were similar to Enterobacter cloacae, and another resembled Enterobacter aerogenes. The characteristics of the fourth strain were similar to those of Klebsiella planticola (Bagley et al., Curr. Microbiol. 6:105-109, 1981). The results of this investigation suggest that the acetylene reduction activity of lenticellate warts of mangrove trunk bark is due to the presence in the warts of nitrogen-fixing bacteria belonging to the family Enterobacteriaceae.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gotto J. W., Taylor B. F. N(2) Fixation Associated with Decaying Leaves of the Red Mangrove (Rhizophora mangle). Appl Environ Microbiol. 1976 May;31(5):781–783. doi: 10.1128/aem.31.5.781-783.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson A. D., Barber L. E., Tjepkema J., Russell S. A., Powelson R., Evans H. J. Nitrogen fixation associated with grasses in Oregon. Can J Microbiol. 1976 Apr;22(4):523–530. doi: 10.1139/m76-078. [DOI] [PubMed] [Google Scholar]
- Pedersen W. L., Chakrabarty K., Klucas R. V., Vidaver A. K. Nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in Nebraska. Appl Environ Microbiol. 1978 Jan;35(1):129–135. doi: 10.1128/aem.35.1.129-135.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raju P. N., Evans H. J., Seidler R. J. An asymbiotic nitrogen-fixing bacterium from the root environment of corn. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3474–3478. doi: 10.1073/pnas.69.11.3474. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright S. F., Weaver R. W. Enumeration and identification of nitrogen-fixing bacteria from forage grass roots. Appl Environ Microbiol. 1981 Jul;42(1):97–101. doi: 10.1128/aem.42.1.97-101.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zuberer D. A., Silver W. S. Biological dinitrogen fixation (acetylene reduction) associated with Florida mangroves. Appl Environ Microbiol. 1978 Mar;35(3):567–575. doi: 10.1128/aem.35.3.567-575.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]