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. Author manuscript; available in PMC: 2008 May 23.
Published in final edited form as: Oncogene. 2003 May 22;22(21):3221–3230. doi: 10.1038/sj.onc.1206381

Figure 1.

Figure 1

Expression of SOCS-1, SOCS-3 and CIS mRNA in normal erythroid progenitors, (a) Cytocentrifuge preparations of splenic erythroid cells isolated from phenylhydrazine-treated mice stimulated in vitro for 0, 24 or 48 h with Epo (5 U/ml). (b) mRNA was isolated from splenocytes and subjected to RT-PCR analysis for 25, 30 or 35 cycles using primers specific for SOCS-1, SOCS-3, CIS and β-actin. (c) PCR products for SOCS-1, SOCS-3 and CIS were quantitated using NIH Image 1.6 and plotted relative to β-actin levels