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. Author manuscript; available in PMC: 2008 May 23.
Published in final edited form as: Oncogene. 2003 May 22;22(21):3221–3230. doi: 10.1038/sj.onc.1206381

Figure 2.

Figure 2

Expression of SOCS-1, SOCS-3 and CIS mRNA in erythroleukemic cell lines, (a) Flow cytometry was used to characterize cell surface expression of c-Kit, Epo receptor (Epo-R), transferrin receptor (Tf-R) and Ter119 on erythroleukemic cell lines, (b) [3H]thymidine incorporation of unstimulated (white histogram) or Epo-stimulated (5U/ml; black histogram) cells is expressed as a per cent relative to unstimulated cells, (c), Northern analysis of poly A+ RNA from cells stimulated with Epo (5U/ml) for 0–6h. Membranes were probed with SOCS-1, followed by CIS and then GAPDH. Independent membranes were probed with SOCS-3 and GAPDH