Figure 5.

Reduced cell growth and survival by CXB, UMC, and DMC in tumor cells with high or no COX-2 expression. MIA-PaCa-2 (COX-2 negative) and Bx-PC-3 (high COX-2 expression) pancreatic carcinoma cells were treated with the indicated concentrations of CXB, UMC, or DMC. After 48 hours, MTT assays were performed to indicate the overall viability in response drug treatment. The right panels show representative 96-well plates of treated cells. Deep purple indicates fully viable cell cultures, whereas yellow color reveals drug cytotoxicity (each condition was tested in duplicate). The single well labeled "Bg." represents background of medium alone without cells. The charts on the left present the quantitative readout of the optical density of the 96-well plates (average of two wells each). The whole experiment was independently repeated and yielded very similar outcomes.