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. Author manuscript; available in PMC: 2008 May 24.
Published in final edited form as: Br J Haematol. 2007 May 9;137(6):578–590. doi: 10.1111/j.1365-2141.2007.06597.x

Fig 2.

Fig 2

Germinal centre B-cell expressed transcript 2 (GCET2) is localised in the cell membrane but is excluded from the lipid rafts. (A) GCET2 is constitutively localised in the plasma membrane. The cell membrane (M) and the cytosol (C) were separated from DHL16 cells transduced with pMIG-GCET2. The upper panel was blotted using anti-V5 antibody to detect the exogenously expressed GCET2, and the second panel was blotted using anti-GCET2 antibody to detect the endogenous GCET2. CD20 and GRB2 were used as markers for the plasma membrane and cytosolic fractions, respectively. (B) GCET2 is excluded from the lipid rafts of cell membrane. GCET2 is not detected in the fractions 4–6 of the sucrose gradient, which represent the lipid rafts, before or after pervanadate treatment. LYN, known to be localised in the lipid rafts, was used as the positive control.