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. Author manuscript; available in PMC: 2008 May 24.
Published in final edited form as: Br J Haematol. 2007 May 9;137(6):578–590. doi: 10.1111/j.1365-2141.2007.06597.x

Fig 4.

Fig 4

Germinal Centre B-cell Expressed Transcript 2 (GCET2) membrane localisation is mediated through myristoylation and palmitoylation. DHL16 cells were labelled with myristic acid [9, 10-³H (N)] or palmitic acid [9, 10-³H (N)]. GCET2 was both myristoylated (A) and palmitoylated (B). Myristoylation is a irreversible process that modifies one amino acid (Glycine-2) of GCET2, while palmitoylation is a reversible process that modifies two amino acids of GCET2 (Cysteine-46 and -48), and GCET2 in the lower molecular weight band (B) may represent the form that is not fully palmitoylated. (C) COS7 cells transfected with wild-type pcDNA3.1-GCET2 with V5 tag. COS7 cells were stained using FITC-labelled anti-V5 antibody. GCET2 protein is predominantly localised on the cell membrane; D. COS7 cells were transfected with GCET2 mutated in the myristoylation site. GCET2 had increased distribution in the nuclei; E. COS7 cells were transfected with GCET2 mutated in the myristoylation and the palmitoylation sites. GCET2 showed nuclear but not membranous distribution.