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. Author manuscript; available in PMC: 2008 May 24.
Published in final edited form as: Br J Haematol. 2007 May 9;137(6):578–590. doi: 10.1111/j.1365-2141.2007.06597.x

Fig 5.

Fig 5

Germinal centre B-cell expressed transcript 2 (GCET2) is phosphorylated after PV Treatment of DHL16 Cells and Daudi Cells. (A) V5 tagged GCET2 was transiently transfected into DHL16 cells. Upon pervanadate treatment, phosphorylated GCET2 migrated to a band of higher molecular weight that reacted with 4G10. (B) GCET2 was phosphorylated in Daudi cells after pervanadate treatment. (C) BCR cross-linking cannot induce GCET2 phosphorylation in DHL16 cells. Both GCET2 and BLNK are phosphorylated upon pervanadate treatment, but BCR cross-linking can only induce BLNK phosphorylation. GCET2 cannot be co-precipitated with BLNK upon pervanadate treatment. Lane 1, DHL16 without treatment; 2, BCR cross-linking; 3, pervanadate treatment. (D) GCET2 is phosphorylated when co-transfected with LYN, LCK or SYK in COS7 cells. Anti-V5 antibody was used for immunoprecipitation (IP) followed by immunoblotting using the anti-phospho-tyrosine antibody, 4G10.