Skip to main content
. Author manuscript; available in PMC: 2008 May 24.
Published in final edited form as: Br J Haematol. 2007 May 9;137(6):578–590. doi: 10.1111/j.1365-2141.2007.06597.x

Fig 7.

Fig 7

(A) Germinal centre B-cell expressed transcript 2 (GCET2) associates with GRB2 following pervanadate treatment. GCET2 is phosphorylated (first panel) and a weak signal of GRB2 is detected in the immunoprecipitates with V5 antibody (second panel). A strong signal of GCET2 is detected in the immunoprecipitates with GRB2 antibody (fourth panel). (B) The third tyrosine of GCET2 is important for its association with GRB2. Single mutants of GCET2 (M1 to M5) were transfected into COS7 cells together with LYN and SYK, and then anti-GRB2 antibody was used to precipitate GCET2. M3 has no association with GRB2. M1 and M2 had strong association to GRB2. M4 and M5 had slight decrease of association. IP, immunoprecipitation, IB, immunoblotting.