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. Author manuscript; available in PMC: 2008 Jun 6.
Published in final edited form as: Oncogene. 2003 May;22(19):2950–2959. doi: 10.1038/sj.onc.1206423

Figure 4. Transduction of myeloma cells with Mcl-1-GFP retrovirus or control GFP retrovirus.

Figure 4

A: XG-1 and XG-6 myeloma cells were transduced with Mcl-1-GFP or GFP retroviruses and were selected with 700 μg/mL G418 and 2 ng/mL IL-6. All selected myeloma cells highly expressed GFP after 15 days of culture.

B: Parental XG cells, XG cells transduced with Mcl-1-GFP or GFP retroviruses were cultured with IL-6 and harvested for Western blot analysis. Blots were probed with anti-Mcl-1, anti-Bcl-xL or anti-β actin antibodies. Western blots are for one representative experiment out of three.