Figure 7. Transduction with Mcl-1 retrovirus obtains reproducible myeloma cell lines. surviving IL-6 starvation.
A: The 5 XG-6GFP and XG-6Mcl-1 clones were obtained in five separate transduction experiments with GFP or Mcl-1-GFP retroviruses starved of IL-6 for 1 hour and cultured for 3 days without cytokine. At day 3 of culture, the percentage of apoptotic cells were determined by staining with annexin V. Statistical analysis was performed with a Wilcoxon test.
B: XG-6 cells transduced with GFP or Mcl-1-GFP retroviruses were cultured at 105 cells/mL with or without 2 ng/mL of recombinant IL-6. Every 3–4 days, cells were counted and diluted at 105 cells/mL with fresh culture medium containing the initial cytokine concentration. Results are the cumulative cell numbers obtained from 18 days of culture. They are those of one experiment representative of two.