Figure 1.

Comparison of the expression fold-changes of selected genes measured by microarray and quantitative RT-PCR. Of 11 pairs of RNA samples used in microarray analysis, only 6 pairs had enough RNA for quantitative RT-PCR analysis, including 5 patients with acute respiratory distress syndrome (ARDS) and 1 control subject. Gene PI3, IL8, and HPGD were chosen as having large differential expression between acute stage and stable stage of ARDS, as well as being identified repeatedly in Medline literature mining. Gene CYP4F3 with marginal expression change and gene MMP9 with no change were chosen as reference genes in validation. The results of gene ACTB was also illustrated, which was originally selected as endogenous control. The y-axis displays the fold change of the recovery stage to the acute stage of ARDS.