TABLE 1.

CHEMOKINE RECEPTORS PREDICTED TO BE TYROSINE SULFATED

Common Name	Swiss-Prot Name	Accession #	Tyrosine #	Sequence	Score
CXCR1	CXCR1_HUMAN	P25024	27	padedYspcml	4.13
CXCR2	CXCR2_HUMAN	P25025	197	vspacYedmgn	1.80
CXCR3	CXCR3_HUMAN	P49682	27*†	nfsssYdygen	2.94
			29*†	sssydYgenes	7.97
CXCR4	CXCR4_HUMAN	P30991	12	ytsdnYteemg	5.06
			21*	mgsgdYdsmke	7.72
CXCR6	CXCR6_HUMAN	O00574	6†	maehdYhedyg	8.19
			10†	dyhedYgfssf	5.59
CCR1	CCR1_HUMAN	P32246	10‡	nttedYdttte	5.13
			18‡	ttefdYgdatp	7.31
CCR2b	CCR2_HUMAN	P41597	26*†	ttffdYdygap	7.55
			28†‡	ffdydYgapch	3.55
CCR3§	CCR3_HUMAN	P51677	17	gttsyYddvgl	3.45
			172	pefifYeteel	2.01
CCR4	CCR4_HUMAN	P51679	22	snyylYesipk	1.48
			284	eryldYaiqat	2.51
CCR5	CCR5_HUMAN	P51681	3*	…mdYqvssp	2.51
			10*†	vsspiYdinyy	2.59
			14*†	iydinYytsep	2.78
			15*†	ydinyYtsepc	3.36
			187	fpysqYqfwkn	2.42
CCR6	CCR6_HUMAN	P51684	18	dssedYfvsvn	1.72
CCR8§‖	CCR8_HUMAN	P51685	15†‡	ttvtdYyypdi	3.74
			17†‡	vtdyyYpdifs	2.87
CCR9	CCR9_HUMAN	P51686	17	nmaddYgsest	5.60
			28	ssmedYvnfnf	2.25
CCR10	CCR10_HUMAN	P46092	22	deedaYsaepl	3.58
CX3CR1	CX3C1_HUMAN	P49238	14*	tenfeYddlae	8.97
XCR1	XCR1_HUMAN	P46094	14	sttffYydlqs	2.32

^*Tyrosines that have a demonstrated presence of O-sulfate on one or more tyrosines. The scores of these tyrosines, whose sites are incorporated into the PSSM, are used as a standard for comparison.

^†Predicted tyrosine sulfation sites that exhibit clustering.

^‡N-terminal peptides have been shown to be sulfated by TPST1 and TPST2 in a cell-free system (44)

^§Protein has been previously shown to be tyrosine sulfated through fusion with heavy chain of IgG1 (5).

^‖Murine CCR8 is known to be sulfated at tyrosines 14 and 15.