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. 2008 Jun 4;3(6):e2366. doi: 10.1371/journal.pone.0002366

Figure 4. Residual granule cell precursors in the cerebellar cortex of peripuberal rabbits.

Figure 4

Neuroblasts forming chains in the SPL are immunoreactive for PSA-NCAM (A), DCX (A,B,E), and Map5 (E), partially for Tag1 (B,C) and NeuN (F), but not for CRMP-4 (D). NeuN (F,G) and Pax6 (H–K) reveal residual granule cell precursors both in the SPL and in the ML, but they are not present in all DCX+ cells (J). See also Table in (L); (1), Overlapping populations; red lines, antigens revealing subpopulations of (1). (2), Antigens whose immunoreactivity is present/absent in a subpopulation of bipolar cells (their counterpart being represented in Figure 2L). (M), Cell death revealed with Tunel technique is reduced in the shift from the third to the fourth month of age (3 m, 4 m), suggesting that granule cell genesis is coming to an end. Student's T-test revealed a significant decrease of the number of tunel labelled cells in each layer (SPL p = 0,010; ML p = 0,005; GL p = 0,004 WM p = 0,013).This is confirmed by the fact that Pax6 and NeuN are no more detectable after the fourth postnatal month (N). Bars: 10 µm.