Figure 4.

Properties of mut2 channels activated by AcCho (Left) or strychnine (Right), in one oocyte injected with mut2 cDNA. (A) Macroscopic voltage-clamped currents. Holding potential, −100 mV. (B) Single-channel currents elicited by AcCho and strychnine in one outside-out patch. Dashed lines indicate amplitude levels of elementary inward currents (downward deflections). Holding potential, −50 mV. (C) Distributions of single-channel amplitudes, detected from recordings shown in B and best-fitted by the sum (thick line) of two Gaussian functions. Unitary conductances as indicated.