Figure 7.
AcCho dose/IAcCho response relationship best-fitted to a sum of two Hill equations in oocytes injected with a mixture of cDNA encoding mut1 and mut2 subunits with ratio of 1:5. The EC50, nH, and weight values determined from the fit were: 1 μM, 2, and 55%, respectively (first component), and 25 μM, 2.5 and 45%, respectively (second component). The peak AcCho currents were normalized to that evoked by 100 μM AcCho (IAcCho mean amplitude: −1.4 μA). Each point represents the mean ± SEM (6/2). A single Hill equation fit was statistically less significant (F-distribution test). (Inset) Sample IAcCho.