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. Author manuscript; available in PMC: 2008 May 26.
Published in final edited form as: Biochemistry. 2007 Jun 13;46(27):8121–8127. doi: 10.1021/bi602418e

Figure 2.

Figure 2

Secondary plots showing the iron dependence of the observed kinetic parameters. The points are the apparent kinetic parameters that arise from fitting the Michaelis-Menten equation to individual vi vs. [S] curves and the lines are described by equation 1 (Vapp filled squares, V/Kapp filled circles) with characterizing parameters, (top) for R115L ferrochelatase KmFe 7.7 ± 1. 4 μM, KmDIX 5.5 ± 0.5 μM, KFe.DIX 30.54 ± 8.01 μM2, V 1.21 μMmin−1 and (bottom) R115L, E343D ferrochelatase KmFe 8.2 ± 1.0 μM, KmDIX 5.8 ± 0.8 μM, KFe.DIX 34.8 ± 8.3 μM2, V 0.16 ± 0.01 μMmin−1.