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The dependence of kobs for the reaction of R115L human ferrochelatase (0.5 μM) on Fe2+ concentration at pH 8.1, 100mM TRIS-HCl, 50 μM DIX, 1mM β metcaptoethanol, 0.5% (v/v) Tween 20, 30°C. The points are experimental and the theoretical line is described by equation 3 and characterizing parameters kchel 0.96 ± 0.05 s−1 and KsFe 79.1 ± 11.2 μM.
