Figure 3.

Loss of Ysl2p severely affects the subcellular distribution of HA–Gga2p and HA–Gga1p. Indirect immunofluorescence was performed with wild-type (wt) and Δysl2 cells expressing either HA–Gga2p (A) or HA–Gga1p (C) as described in Figure 2. Relative to HA–Gga2p, images of HA–Gga1p staining were obtained with three-fold longer exposure time. (B) Cell extracts of wt and Δysl2 cells expressing HA–Gga2p or HA–Gga1p were analysed by immunoblotting with an anti-HA and anti-PGK antibody. (D) Wild-type and Δysl2 cells expressing plasmid-encoded Myc–Tlg1p were stained with an anti-c-Myc antibody. (E) Δysl2 cells transformed with empty vector or the indicated plasmids were stained for HA–Gga2p. Bar, 5 μm.