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. 2008 Apr 17;27(10):1481–1490. doi: 10.1038/emboj.2008.76

Figure 2.

Figure 2

Hzf is required for efficient C/EBPα expression and adipogenesis in fibroblasts. (A) Control, sh-Hzf, and sh-SCR 3T3-L1 cells were stimulated to differentiate for indicated periods, and expression of the indicated proteins was analysed by immunoblotting with CDK4 as a loading control. Values represent the ratios of PPARγ and C/EBPα to CDK4 protein. (B) 3T3-L1 cells infected with indicated retroviruses were induced to differentiate for 6 days, and triglycerides in cell lysates were measured. (C) Differentiated 3T3-L1 cells infected with indicated retroviruses were assayed for 2-deoxyglucose uptake in the presence of 5 μg/ml of insulin.