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Exogenous C/EBPα restores adipocytic differentiation of sh-Hzf-treated 3T3-L1 cells. Cells expressing the indicated shRNAs and co-infected with retroviral vectors encoding C/EBPα+GFP or GFP alone were stimulated to differentiate for indicated periods. Protein expression was analysed by immunoblotting (A), and cells cultured in differentiation-inducing media (4 days) were stained with Oil Red O (B). Values represent the ratios of PPARγ protein to CDK4 protein in each sample.
