Figure 5.

Signal end complexes formed by Rag1 mutants, but not Rag2 K38A/R39A, are able to capture target. (A) Rag-signal end complexes were formed in vitro and then incubated with radiolabeled GC hairpin target. After 2 h, complexes were separated on a native 5% polyacrylamide gel, which was visualized by autoradiography. (B) Rag-signal end complexes were formed and then incubated with radiolabeled AC hairpin target. After 2 h, complexes were separated on a native 5% polyacrylamide gel, which was visualized by autoradiography. TCC, target capture complex.