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. 2008 Apr 4;36(9):3011–3024. doi: 10.1093/nar/gkn150

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Tip60 enhances Sox9 transcriptional activity. (A) Tip60 enhances Sox9 transactivation activity using a reporter with a multimerized Col2a1 enhancer (4 × 48 Col2a1 p89). COS7 cells were cotransfected with HA3-Sox9 (3 ng) and/or GFP-Tip60 (1 μg) expression vectors with 4 × 48 Col2a1 p89 (0.77 μg), or the inactive mutant mA6 of 4 × 48 Col2a1 p89 (0.77 μg), or with p89 (0.77 μg) luciferase reporter constructs and SV40/β-galactosidase (3 ng) reporter construct as indicated. (B) Tip60 enhances Sox9 transactivation activity of a reporter with a multimerized Col2a1 enhancer (4 × 48 Col2a1 p89) dose dependently. Cos7 cells were cotransfected with HA3-Sox9 (100 ng) and/or GFP-Tip60 (0.5 or 1 μg) expression vectors together with 4 × 48 Col2a1 p89 (0.77 μg) luciferase reporter construct and SV40/β-galactosidase (3 ng) reporter construct as indicated.