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. 2008 Apr 4;36(9):3011–3024. doi: 10.1093/nar/gkn150

Figure 5.

Figure 5.

Subnuclear co-localization of Sox9 with Tip60 in the nucleus. (A) Sox9 protein and Tip60 protein in HCS-2/8 cells were stained with anti-Sox9 (green) and Tip60 (red) antibodies, respectively. The subnuclear localization of proteins was detected by indirect immunofluorescence and analyzed by deconvolution microscopy. The subnuclear distribution of endogenous Sox9 and Tip60 in HCS-2/8 cells overlapped in a diffuse staining pattern. (B–D) COS7 cells were transfected with expression vectors encoding HA3-Sox9 (B), or GFP-Tip60 (C) or both (D). Sox9 was stained with anti-Sox9 antibody and detected by indirect immunofluorescence, and Sox9 and Tip60 were analyzed by deconvolution microscopy. Sox9 alone displayed a punctuated distribution in the nucleus (B), while Tip60 alone showed a somewhat patchy distribution in the nucleus (C), but the Tip60 distribution became more diffused in the presence of Sox9 (D); about 50% of the nuclear area shows codistribution of Sox9 and Tip60.