Figure 3.

Alternative initiation of ARFP translation in the +1 frame. (A) Plasmid constructs containing the *S₂₂ stop codon mutation in the +1 frame resulting in knock-out of fARFP expression. Plasmid numbers are indicated. (B) Potential proteins resulting from translation in the 0 and +1 frames. (C) Huh-7 cells were transfected with the constructs shown in (A) and luciferase activity was assessed as described above. Black and grey bars correspond to HCV translation in the 0 and +1 frame, respectively.