Table 1.
Summary of results for qRT–PCR amplifications using the first two selected pairs of MultiPriDe-developed primers
| Species | Successful qRT–PCR reactions at (200 nM) | Total successful qRT–PCR reactions | Total genes analyzed |
|---|---|---|---|
| Human (%) | 39 (53) | 55 (74) | 74 |
| Mouse (%) | 61 (75) | 71 (88) | 81 |
| Rat (%) | 43 (45) | 63 (66) | 96 |
| All species | 143 (57) | 189 (75) | 251 |
‘Successful qRT–PCR reactions at (200 nM)’ includes only those reactions that were successful at 200 nM primer concentration. ‘Total successful qRT–PCR reactions’ includes those successful at 200 nM, as well as those that were not successful at 200 nM, but were subsequently successful when tested at 100 nM or 400 nM. Percentages are calculated based on total genes analyzed for a given species.