Figure 1.

The CYC1 promoter of the transcription templates used in this work. (A) Above is shown the double-stranded sequence of the CYC1 promoter fragment fused to the G-less cassette in the original pJJ470 plasmid (40). Potential transcriptional start sites (41,65,66) located in the G-less cassette are indicated by dots. Below is the double-stranded sequence of the modified CYC1 promoter from the pJJ470(MA) plasmid. Mutations in the TATA-like sequences are shown in bold. (B) List of the wild-type and all 23 TATA box mutants that were used in this study. Whether the sequence was a consensus TATA box and/or TBP sensitive were based on a previous study (4,53). TBP-sensitive promoters show decreased transcription in yeast strains with mutations in the DNA-binding surface of TBP (53).