Figure 3.

Certain nonconsensus TATA boxes facilitate high rates of transcription. Shown are basal transcription activities of wild-type and mutant CYC1 TATA boxes in a purified system in vitro. Mutated bases are shown in bold. Lanes 2 and 4 show nonconsensus TATA boxes with high transcription signals. Lanes 3 and 5 serve as controls and show nonconsensus TATA boxes that give low transcription signals. Basal transcription was measured in a G-less cassette assay that was performed in the presence of highly purified RNA Pol II, TBP, TFIIB, TFIIE, TFIIF and TFIIH.