Figure 2.

dJARID1/Lid demethylates H3K4me3. (A) A Lid-Flag fusion protein was over-expressed in Drosophila S2-cells and transfected cells were stained with αFlag (shown in green), and αH3K4me3, αH3K9me3, αH3K27me3 or αH3K36me3 antibodies (shown in red). DNA was stained with DAPI (shown in blue). Arrows indicate cells over-expressing dJARID1/Lid. (B) Polytene chromosomes from control wild-type larvae (+/+) and homozygous lid¹²³⁶⁷/lid¹²³⁶⁷ mutant larvae stained with αH3K4me3 are shown. DNA was stained with DAPI.