Table 2.
WT and H235N M.EcoRI target base restacking kinetics of cognate DNA
| Enzyme | Amp 1 (%) | Rate 1 (s−1) | Amp 2 (%) | Rate 2 (s−1) |
|---|---|---|---|---|
| WT M.EcoRI | 38 | 0.089 | 62 | 0.022 |
| H235N M.EcoRI | 27 | 0.021 | 73 | 0.0054 |
WT and H235N M.EcoRI target base restacking kinetics of cognate DNA
| Enzyme | Amp 1 (%) | Rate 1 (s−1) | Amp 2 (%) | Rate 2 (s−1) |
|---|---|---|---|---|
| WT M.EcoRI | 38 | 0.089 | 62 | 0.022 |
| H235N M.EcoRI | 27 | 0.021 | 73 | 0.0054 |