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. Author manuscript; available in PMC: 2009 Apr 25.
Published in final edited form as: Virology. 2008 Jan 22;374(1):1–10. doi: 10.1016/j.virol.2007.11.023

Figure 3. The effect of serine-to-alanine mutations on HSV-2 UL13 autophosphorylation and phosphorylation of exogenous substrates.

Figure 3

(A) Overlapping ERK and Cdc2 phosphorylation recognition motifs of HSV-2 UL13. S118 (bold) is the phosphoacceptor for both motifs. HA-tagged UL13 and UL13 mutants were subjected to in vitro kinase assays (B) in the absence of an exogenous substrate (*P = < 0.03 to *** = < 0.001 relative to WT UL13), or (C) in which the exogenous substrate myelin basic protein was added (**P = < 0.01 relative to WT UL13; see Methods for statistical analyses). Data are the mean ± SD from three experiments. Representative phosphorimages are shown for experiments denoted in 3B (D and E) and 3C (F).