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. Author manuscript; available in PMC: 2009 Apr 25.
Published in final edited form as: Virology. 2008 Jan 22;374(1):1–10. doi: 10.1016/j.virol.2007.11.023

Figure 7. HSV-2 UL13 is a proline-directed kinase.

Figure 7

(A) The UL13-WT peptide and mutant peptides were used at 1mM as substrates for ERK in an in vitro kinase assay. Values are the average of 2 experiments. (B) The UL13-WT peptide or mutant peptides were used at 1mM as substrates for Cdc2 in an in vitro kinase assay. Values are the average of 2 experiments ± the range. (C) UL13-HA was expressed by in vitro transcription/translation, immunoprecipitated, and subjected to an in vitro kinase assay for 30 min using the UL13-WT peptide or UL13-WT mutant peptides as substrates. Values were normalized to UL13 phosphorylation of the UL13-WT peptide at 2mM. The data are the average of three experiments ± SD.