Table 1.

Levels of immunogold pLIMK in all synaptic compartments in young and aged estrogen- and vehicle-treated rats. The numbers are the average number of pLIMK immunolabeled gold particles per synapse in different compartments. (*) indicates; E increased number of pLIMK immunoreactive gold particles in the non-synaptic portion of the spine (> 60 nm) in young animals (p = 0.05), but similar effect was not observed in aged animals. The age-by-treatment interaction showed a similar response in the non-synaptic portion of the spine (> 60 nm) but failed to reach a p value of 0.05 (p = 0.08). The numbers are given as mean ± SEM. OVX, ovariectomized; E, 17-B estradiol treated; Veh, vehicle treated.

Bin Designation	Young OVX + E (N=5)	Young OVX + Veh (N=6)	Aged OVX + E (N=6)	Aged OVX + Veh (N=6)
Postsynaptic 0–30 nm	2.68 ± 0.22	2.68 ± 0.19	2.21 ± 0.12	2.49 ± 0.16
Postsynaptic 30–60 nm	1.01 ± 0.12	0.86 ± 0.04	0.89 ± 0.11	1.13 ± 0.09
Postsynaptic >60 nm	2.06 ± 0.34	1.14 ± 0.18*	1.42 ± 0.27	1.39 ± 0.12
Cleft	0.42 ± 0.12	0.42 ± 0.09	0.26 ± 0.03	0.39 ± 0.10
Presynaptic 0–30 nm	0.60 ± 0.08	0.63 ± 0.16	0.52 ± 0.08	0.50 ± 0.08
Presynaptic 30–60 nm	0.30 ± 0.02	0.30 ± 0.08	0.29 ± 0.04	0.21 ± 0.04
Presynaptic >60 nm	1.96 ± 0.36	1.57 ± 0.21	1.54 ± 0.37	1.63 ± 0.26