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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Nutr Biochem. 2007 Sep 27;19(6):400–408. doi: 10.1016/j.jnutbio.2007.06.002

Fig. 3.

Fig. 3

Expression of SMVT decreases in response to biotin supplementation in wild-type cells, but down-regulation is impaired in HCS-deficient cells. (A) Abundance of SMVT mRNA was quantified by real-time PCR. Values are means±S.D. a,b,cBars not sharing the same superscript are significantly different in cells matched for HCS expression. *P<.05, significantly different from knockdown cells matched for biotin (n=3). (B) SMVT protein was probed by Western blot analysis. Ctrl. indicates control (blot probed with secondary antibody in the absence of anti-SMVT). anti-SMVT and Ctrl. samples were run on the same gel, but the Ctrl. lane was processed separately after electroblotting.