Table 2.
Second round of expression screening as fusion proteins.
| ORF | M.W. (kDa) | Fusion partner | cloned | IB* | S | M |
| Rv0011c | 10.2 | GST | Yes | XX** | ||
| Rv0460 | 8.1 | GST | Yes | XX | ||
| Rv0544c | 9.7 | GST | Yes | X | ||
| Rv0882 | 9.6 | GST | Yes | X | ||
| Rv1171 | 15.2 | GST | Yes | |||
| Rv2128 | 7.4 | GST | Yes | X | ||
| Rv3078 | 14.1 | GST | Yes | |||
| Rv3632 | 13.1 | GST | Yes | |||
| Rv3656c | 7.1 | GST | Yes | XXX | ||
| Rv3901c | 15.4 | GST | Yes | XXX | ||
| Rv0011c | 10.2 | MBP | Yes | XXX | XXX | |
| Rv0460 | 8.1 | MBP | Yes | XX | XX | |
| Rv0544c | 9.7 | MBP | Yes | XX | XXX | |
| Rv0882 | 9.6 | MBP | Yes | X | XXX | |
| Rv1171 | 15.2 | MBP | Yes | X | X | |
| Rv2128 | 7.4 | MBP | Yes | XXX | XXX | |
| Rv3078 | 14.1 | MBP | Yes | |||
| Rv3632 | 13.1 | MBP | Yes | X | X | |
| Rv3656c | 7.1 | MBP | Yes | XXX | ||
| Rv3901c | 15.4 | MBP | Yes | XX | XXX | |
| Rv0011c | 10.2 | KSI | Yes | XX | ||
| Rv0460 | 8.1 | KSI | Yes | X | ||
| Rv0544c | 9.7 | KSI | Yes | |||
| Rv0882 | 9.6 | KSI | Yes | |||
| Rv1171 | 15.2 | KSI | Yes | X | ||
| Rv2128 | 7.4 | KSI | Yes | |||
| Rv3078 | 14.1 | KSI | Yes | X | ||
| Rv3632 | 13.1 | KSI | Yes | X | ||
| Rv3656c | 7.1 | KSI | Yes | X | ||
| Rv3901c | 15.4 | KSI | Yes | |||
* IB: Inclusion body fraction; S: Soluble fraction; M: Membrane fraction.
** Symbol "X" indicates the estimated yield of the fusion protein from Coomassie blue stained SDS-PAGE gel. One "X" corresponds to the expression yield between 10 mg and 20 mg for the fusion protein from 1 L LB culture.