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Detecting a single di-nucleotide repeat difference. Two closely-related rootstocks (5C and SO4) share one allele size (259 bp) and differ by a single CT repeat at the other allele (236 bp for 5C and 234 bp for SO4) for microsatellite locus VVMD32. Five individual extractions for 5C and SO4 show the differentiation of these two rootstocks
